Chromatography

Gas Chromatography (GC or GLC)

Gas Chromatography (GC or GLC) is a commonly used analytic technique in many research and industrial laboratories for quality control as well as identification and quantitation of compounds in a mixture. GC is also a frequently used technique in many environmental and forensic laboratories because it allows for the detection of very small quantities. A …

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The Use of Thin Layer Chromatography in the Examination of Analgesic Medication

Component Analysis of Common Analgesic Tablets by Thin-Layer Chromatography Introduction Chromatography is an all-inclusive term that encompasses a set of laboratory methods used to separate mixtures. In this lab the objective was to determine the active ingredients in common analgesic tablets through thin-layer chromatography. To do this a thin-layer chromatography (TLC) plate was utilized. This …

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The combination is size-exclusion chromatography (SEC)

Another example of a combination is size-exclusion chromatography. SEC [bw1] is often used for (bio)polymers. This separation technique is based on the size of the analytes. The separation only depends on the stationary phase, unlike other chromatographic techniques. To be more precise, the separation only occurs in the pores in the packaging material. This is …

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Examination of the Use of Column Chromatography for Spinach Purification

Absorbance Spectra of Spinach Fractions Purified by Column Chromatography Introduction Column chromatography is a particular branch of chemical purification, by which the components of a substance are separated from each other based on each component’s chemical interactions with certain filters – absorbents and solvents. The analyzed species, often a solid ground into a particulate solute, …

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